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Reduction of diacetyl levels by expression of ATP3-5. Fermentations were carried out at 30°C with the rho− strain RKY2399 transformed with plasmid pRK1107 (containing the ATP3 gene, ○) or plasmid pRK1108 (containing the ATP3-5 gene, ▴). Diacetyl levels were determined at the times indicated. (A) SD/CAS medium; (B) MEX medium. Experiments were performed at least three times. The results of a representative experiment are shown.
