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Inner membrane translocation of glycoproteins. Western blot analysis of AcrA-4× targeted to the Tat (spTorA), SRP (spDsbA), or Sec (spMBP) protein export pathway. Proteins were purified from the periplasmic fraction of cells by Ni-NTA affinity chromatography. The same amount of protein was loaded in each lane. Blots were probed with anti-His (a) or hR6P (b) antibodies.
