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Localization of N-glycoproteins in OMVs. Western blot analysis of ClyA-GT in OMV fractions or cell lysates isolated from CE8032 cells carrying pACYCpgl (+) or pACYCpglmut (−). For cell lysates, proteins were Ni-NTA affinity purified. The same amount of protein was loaded in each lane. Blots were probed with anti-His (a) or hR6P (b) antibodies.
