TABLE 2.
Intracellular pHs and total intracellular acetic acid concentrations for the acid-salt treatments used in this study
| NaCl concn (%) and protonated acetate concn (mM) | Calculated extracellular acetate anion concn (mM)a | Intracellular pH (SE)b | Calculated intracellular acetate anion concn (mM)c |
|---|---|---|---|
| 2 | |||
| 0 | 0.00 | 6.35 (0.03) | 0.00 |
| 5 | 0.78 | 6.25 (0.03) | 154.57 |
| 10 | 1.02 | 6.13 (0.02) | 233.22 |
| 20 | 3.13 | 5.93 (0.04) | 298.67 |
| 30 | 4.64 | 5.77 (0.02) | 306.49 |
| 40 | 4.28 | 5.65 (0.06) | 312.34 |
| 4 | |||
| 0 | 0.00 | 5.67 (0.05) | 0.00 |
| 5 | 0.36 | 5.73 (0.03) | 47.17 |
| 10 | 0.71 | 5.97 (0.05) | 162.50 |
| 20 | 1.82 | 5.92 (0.03) | 289.20 |
| 30 | 3.63 | 5.80 (0.05) | 328.24 |
| 40 | 4.31 | 5.71 (0.01) | 353.20 |
a
Calculated from the total protonated acetic acid concentration using high-performance liquid chromatography.
b
Mean intracellular pH from three independent replicates.
c
Calculated using the Henderson-Hasselbalch equation.