TABLE 2.
Characteristics of the biofilm mutants identifieda
| Gene hit | Strain | Phenotype |
||
|---|---|---|---|---|
| Pellicle morphology (thickness score/porosity score/ pigmentation)b | Attachment (mDeg)c | Motility (% of wild type)d | ||
| — | TT01 rif | 5/5/orange | 116 | 100 |
| plu2360 manA | P56 | 1/0/none | 96 | 16 |
| plu4799 wblD | P73 | 4/1/orange | 106 | 89 |
| plu0934 suhB | P36 | 0/0/none | 90 | ND |
| plu4004 diaA | P27 | 0/0/none | 46 | 13 |
| plu2728 entB | P32 | 0/0/none | 103 | ND |
| plu2728 entB | P40 | 0/0/none | 85 | ND |
| plu3085 nuoF | P72 | 4/2/pale | 105 | 80 |
| plu3085 nuoF | P38 | 3/2/pale | 93 | ND |
| plu1849 motA | P79 | 0/0/none | 95 | 0 |
| plu1850 motB | P69 | 0/0/none | 140 | 0 |
| plu1919 flgF | P30 | 0/0/none | 83 | ND |
| plu1920 flgG | P84 | 0/0/none | 100 | 0 |
| plu1851 cheA | P71 | 0/0/none | 67 | 0 |
| plu4890 | P74 | 4/4/white | 133 | 86 |
| plu2203 hcaR | P82 | 3/2/orange | 84 | 105 |
| plu2209 hcaD | P78 | 2/3/white | 79 | 107 |
| plu3559 | P31 | 0/0/none | 93 | ND |
| plu0499 | P75 | 2/0/orange | 88 | 84 |
| plu2244 | P86 | 2/1/orange | 81 | 98 |
| plu2548 | P77 | 2/1/dark orange | 68 | 97 |
| plu1730 | P29 | 0/0/none | 91 | ND |
| plu2412 | P76 | 3/2/red | 116 | 93 |
| plu4230 | P33 | 1/0/orange | ND | ND |
| ND | P80 | 3/3/dark orange | 78 | 100 |
| ND | P85 | 5/5/white | 59 | 110 |
| ND | P89 | 0/0/none | 108 | 15 |
| ND | P88 | 2/2/orange | 108 | 65 |
| ND | P81 | 0/0/none | 123 | 0 |
| ND | P83 | 2/3/dark orange | 96 | 95 |
a
Gene disrupted by transposon, pellicle morphology, attachment ability, and swimming motility. ND, not determined.
b
Scores for pellicle assay to estimate thickness/porosity/pigmentation (arbitrary scale from 0 [none] to 5 [wild-type level]).
c
Surface plasmon resonance measurements are maximum angle variation (mDeg) after 1,000 s.
d
Diameter (percentage of TT01 rif) of swimming halo after 6 days.