TABLE 1.

Details of loci used in the MLST scheme

Gene	Chromosome locationa	Primer no.	Direction	Size (bp)	Oligonucleotide sequence 5′-3′	Annealing temp (°C)	Length of sequenced fragment (bp)	No. of alleles	% Sequence diversity [mean (maximum)]b
mdh	546394-547332	9091	Forward	18	CCCAGCTTCCTTCAGGTT	50-55	607	8	0.95 (3.79)
		9171	Reverse	18	AGCACTCAGTTTACCGAT	50-55
gyrB	4704462-4706876	9169	Forward	18	TGGTATTCGAGGTTGTGG	50-55	627	14	0.99 (3.19)
		9170	Reverse	18	TTCAGGGTACGGGTCATC	50-55
fumC	2574258-2575655	9167	Forward	19	GATGATGTCAATAAAAGCC	50-55	574	13	1.53 (6.1)
		9168	Reverse	18	GCAGTGCTCATTAAAACC	50-55
sucA	1373784-1375007	9173	Forward	18	TTCAATGTCGGTTCTTTT	50-55	611	13	0.88 (2.95)
		9174	Reverse	18	CGCGCATCCAGTGGGTTC	50-55
pgi	4344997-4346643	9144	Forward	18	TTTGCCAAGGACGACCAG	50-55	623	10	0.69 (2.73)
		9145	Reverse	18	CAACCGACAAGGCGATAG	50-55
recA	988499-989569	9172	Forward	18	TGTTGAAACCATCTCTAC	50-55	584	14	1.36 (5.48)
		9147	Reverse	18	TGGCATTGGCTTTACCCT	50-55
aroC	3109434-3110519	9148	Forward	18	GGCATTCCGATTACCGAG	50-55	597	9	1.54 (4.36)
		9166	Reverse	18	GCCGATTGATAGTTTGCC	50-55

^aPositions are based on the Y. pseudotuberculosis strain IP32953 chromosome (9).

^bExcluding strain Y25.