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. 2010 Nov 29;77(3):711–718. doi: 10.1128/AEM.01610-10

FIG. 3.

FIG. 3.

Proline response of ArgR to the upstream regions of the argB gene. (A) DNA-binding activity of His6-ArgR (2,400 nM) for subfragments B1 and B2 by EMSA in the presence of 0 mM, 1 mM, 5 mM, and 10 mM proline. (B) RT-PCR analysis of the argB gene in 10 mM proline-treated C. glutamicum. The mRNA expression level of the argB gene was calculated as a ratio of 16S rRNA gene expression. The results are reported as the means of data from three experiments.