FIG. 3.

Morphology on agar plates of Mycosphaerella graminicola wild-type and transformed strains. Growth morphology on agar plates was tested with the wild-type (WT) strain, the ectopic integration (Ect) strain, and four independent MVE1 gene deletion mutants. All strains were grown on potato-dextrose agar (PDA) or yeast-sucrose agar (YSA) for 15 days. Dark pigmentation was completely absent in the four independent MVE1 mutant isolates (Δmve1-1, Δmve1-2, Δmve1-3, and Δmve1-4), regardless of temperature or growth medium. Locations of inoculated strains on the agar plates are indicated at the bottom of each set of images. The growth temperatures are indicated on the left. RT, room temperature.