FIG. 6.
Defects in development of aerial mycelia of the Mycosphaerella graminicola MVE1 mutants were independent of light or dark conditions. Aerial mycelium was photographed with a dissecting microscope. All strains were grown on water agar at room temperature for 10 days. Each fungal culture was grown under continuous blue- or red-light conditions as well as under temporary blue- or red-light conditions. For temporary blue or red light, each fungal strain was subjected to continuous blue or red light for 2 days and then shifted to complete darkness. The dark condition was established by wrapping fungal cultures with aluminum foil. A specific spectrum of light was allowed to penetrate into the culture boxes through the use of blue or red cellophane windows under an LED lamp. The light conditions of growth on agar plates are indicated on the left: Blue, fungal cultures grown under continuous blue light; Red, cultures grown under continuous red light; Blue to dark, cultures grown under temporary blue light; Red to dark, cultures grown under temporary red light; Dark, continuous darkness. WT, wild type; Ect, ectopic control; Δmve1-1, Δmve1-2, Δmve1-3, and Δmve1-4, four independent MVE1 mutants.