FIG. 8.

The MVE1 mutants of Mycosphaerella graminicola exhibited reduced hydrophobicity. (A) Fungal mat formation test. Each strain was inoculated into yeast-sucrose broth (YSB) with the same amount of fungal cells (initial concentration of 1 × 10⁵ cells ml⁻¹). After 15 days of incubation without shaking, YSB cultures of the wild-type strain, an ectopic transformant (Ect), and two independent MVE1 gene deletion mutants (Δmve1-1 and Δmve1-2) were photographed. The wild type and an ectopic transformant generated a fungal mat on the surface of the liquid medium, whereas the two MVE1 mutants did not. (B) On each of the fungal colonies grown on YSA for 30 days, 15 μl of water-based DNA loading dye was pipetted onto the colony surfaces and photographed 10 min later. Spherical water droplets formed on colonies of the wild type and an ectopic transformant, whereas the droplet dispersed immediately on colonies of the four MVE1 gene deletion mutants (Δmve1). The locations of inoculated strains on the agar plate are indicated at the bottom of the image.