TABLE 3.
Comparison of gene expression ratios between the strain of interest and the reference strain, LD61, in transcriptomic and qPCR analysesa
| Relative gene expression | Gene name | Strain | Transcriptomic ratio | qPCR ratio normalized with: |
|
|---|---|---|---|---|---|
| pbp2A | smc | ||||
| Increased | feoA | UCMA5713 | 3.5 | 5.2 | 6.8 |
| feoA | LD55 | 3.3 | 4.7 | 4.8 | |
| feoA | LL52 | 4.9 | 1.3 | 1.2 | |
| yaiA | LL52 | 5.6 | 2.8 | 2.7 | |
| Unchanged | feoA | LL08 | 1.0 | 1.0 | 1.0 |
| Decreased | ipd | UCMA5713 | 0.1 | 0.2 | 0.3 |
| ipd | LD55 | 0.2 | 0.1 | 0.1 | |
| ipd | LL52 | 0.1 | 0.1 | 0.1 | |
| ipd | S86 | 0.1 | 0.1 | 0.1 | |
| pyrR | LL52 | 0.2 | 0.5 | 0.4 | |
| pyrR | S86 | 0.3 | 0.1 | 0.1 | |
a
Corrected in qPCR experiments by using pbp2A or smc normalization ratios.