TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Genotype or relevant characteristicsa	Source or reference
Strains
P. luminescens
TT01	Strain isolated from the nematode Heterorhabditis bacteriophora THO1 in Trinidad; wild-type form	26
TT01α	Genomic variant of TT01 isolated from the laboratory-maintained nematode Heterorhabditis bacteriophora THO1; wild-type form	31
VAR*	Stabilized TT01α phenotypic and colonial variant	31
TT01ΔsodA	TT01R sodA::cat	17
E. coli
XL1-Blue MRF′	Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac F′ [proAB lacIqZΔM15 Tn10 (Tetr)]	Stratagene
S17-1	pro r− n− Tpr Smr RP4-2-Tc::Mu::Tn7 recA thi	60
Plasmids
pBBR1-MCS1	Broad-host-range vector; Cmr, mob	42
pBB-pit	0.5-kb PCR fragment obtained with XhoI-L-plu1537 and PstI-R-plu1537 primers and inserted between the XhoI and PstI sites of pBBR1-MCS1	This study
pBB-pirAB	1.7 kb PCR fragment obtained with XhoI-L-plu4093 and PstI-R-plu4092 primers and inserted between the XhoI and PstI sites of pBBR1-MCS1	This study
pBB-xnp2	7.3-kb PCR fragment obtained with XhoI-L-plu2444 and PstI-R-plu2441 primers and inserted between the XhoI and PstI sites of pBBR1-MCS1	This study
pBB-UIDK	pBB-uidA; Kmr	46
pBB-PlacuidA	PvuII Plac fragment from pUC19 inserted into pBB-UIDK	This study

^aCm^r, chloramphenicol resistance; Tp^r, trimethoprim resistance; Sm^r, streptomycin resistance; Km^r, kanamycin resistance.