TABLE 2.

Oligonucleotides used in this study

Use and oligonucleotide	Sequence (5′-3′)a	Location
qRT-PCR
L-0004	ATACACGAAGAAGAAGGTGTTTCAG	Internal region within plu0004
R-0004	TACCTGTCTGTTCAGTTTCTCCAAC	Internal region within plu0004
L-0157	ATGAAGAGCTGATTGTCGATGATG	Internal region within plu0157
R-0157	CGTAAAAGAGAAAGATAGAGCAGCAC	Internal region within plu0157
L-0183	AGTGTGGAACGTTGGATAGATAGAG	Internal region within plu0183
R-0183	AAGTATAGCTGCACCTACAGAACAAC	Internal region within plu0183
L-1537	ACTTATCCAGGATTCTATTCGTCTG	Internal region within plu1537
R-1537	ACTATAGATTTCGCCTTTGCTATCC	Internal region within plu1537
L-1752	CCTACGATATAGGCAATGGTATCAG	Internal region within plu1752
R-1752	ACAATATCTTGGTAGCCGAAAGAG	Internal region within plu1752
L-1956	GTACCTGTTTGAACAGACTGAAATG	Internal region within plu1956
R-1956	TTGTAGTTACTGAATGCTGACTGTG	Internal region within plu1956
L-1991	GCTAATATTTCCGTAGGCTCTCATC	Internal region within plu1991
R-1991	GGACGTAATCAAAGTGTAATTCTCG	Internal region within plu1991
L-2442	AATAGGCAATATGCTGTACGTCTTC	Internal region within plu2442
R-2442	CAGACCGCAAATACAATAAAGTAGG	Internal region within plu2442
L-3506	TTAAGGGCGATATTGATGTAGAAGG	Internal region within plu3506
R-3506	TTGATAGACATACTGCTGCAACGAC	Internal region within plu3506
L-4092	GACAGTGACCATGAACAGACATAAC	Internal region within plu4092
R-4092	ATATTGGGTTGCTGGTTTAGAGAG	Internal region within plu4092
L-4546	CTGCCGATATAATTGCAGCTTTAC	Internal region within plu4546
R-4546	CCAAATTTCTGAAGGGTGTATTTCC	Internal region within plu4546
Plasmid constructions
XhoI-L-plu1537	GCGCCTCGAGTTAAAATCAATCACAAGAGG	Upstream of plu1537
PstI-R-plu1537	GCGCCTGCAGAATATCTTCTCACAGGAATGG	Downstream of plu1537
XhoI-L-plu4093	GCGCCTCGAGTTAATGAGGAAAATAAATATGTC	Upstream of plu4093
PstI-R-plu4092	GCGCCTGCAGCTACGTACATAAAATATACTTGTTAAA	Downstream of plu4092
XhoI-L-plu2444	GCGCCTCGAGGAGGCCGATAGCTTCCAAC	Upstream of plu2444
PstI-R-plu2441	GCGCCTGCAGAAAGCGTAGCAATGTTCCTG	Downstream of plu2441

^aRestriction enzyme sites are underlined.