TABLE 2.
Peptides of chlorite dismutase and perchlorate reductase in late log growth phase of Dechloromonas agitata culture
| Protein (avg sequence coverage [%], no. of peptides matched [range], mean concn of peptides [ng/mg of total protein]a) | Signature peptide sequences |
|---|---|
| Chlorite dismutase (66.3, 39-51, 2,454)b | AAEVVAVVEK, FGNFSTYK, PTLANLVNVK, AEAYLTR, FVIPVKK, QSDFFLR, AEAYLTRGFEAQSDFFLR, GAAAEVVAVVEK, SPNLNAGLTGATYR, AEVVAVVEK, GFEAQSDFFLR, SPNLNAGLTGATYRDATPR, AFLVDFR, IHSYDMAATQAFLVDFR, TAPGVFGNFSTYK, AFVIPVK, ILTAPGVFGNFSTYK, TLANLVNVK, AFVIPVKK, ILTAPGVFGNFSTYKVRPDYYK, TQAFLVDFR, ANLVNVK, KGAAAEVVAVVEK, VKAEAYLTR, AQSDFFLR, KNADWWNLTDEQR, VRPDYYK, DKSPNLNAGLTGATYR, LANLVNVK, VVAVVEK, DLNYITK, LKEMETHTLPTLANLVN, WGSPTVLGTIQSFDSVVNTLSMGR, DWWNLTDEQR, LKEMETHTLPTLANLVNVK, YAFVIPVK, EAQSDFFLR, LSMAER, YAFVIPVKK, EMETHTLPTLANLVNVK, NADWWNLTDEQR, YHVRWGSPTVLGTIQSFDSVVNTLSMGR, EVVAVVEK, NLNAGLTGATYRDATPR, YRDATPR, FGMNAEVTENLVGMTK, PGVFGNFSTYK |
| Perchlorate reductase alpha subunit (66, 85-96, 2,511)c | ACPHFVYTK, GACPHFVYTK, PHFVYTK, ADNNIAK, GAHLINCTGACPHFVYTK, PLPGTAPVSFSAGHR, AMHLLTALVGSEGK, GECAHHYMYGPHR, PPMPNIPELNPR, ANWTSPMK, GGPCVEISPIDATAIGVK, PTHLVGNYGHLVFRPNYYGPAGSQR, ANWTSPMKEGVPYTPFQNYIVDK, GITLQMLR, PVAFMGR, APPVAFMGR, GNWLNQAK, RATWEEALDLISDK, APPVAFMGRNTHTFPK, GNWLNQAKGQK, RYIGATPISL, APVDADKFPFR, GSWGDQPEQK, SISPDFNSSTIK, ASAATLLGNSLTFK, GYIALENLD, SPLPGTAPVSFSAGHR, ATWEEALDLISDK, GYIALENLDPALEGK, SRIEADNNIAK, DGVVIR, GYIALENLDPALEGKFQVK, SVYSPLPGTAPVSFSAGHR, DGVVIREEQSK, HSIHSTFK, TDLPYLIR, DIPPMPNIPELNPR, HSIHSTFKDSVLMLR, TDWQIFLALA, DIPPMPNIPELNPRGCNK, IEADNNIAK, TDWQIFLALAK, DKFYIWDSK, IEADNNIAKAAK, TIIEVAR, DLPYLIR, IIDTIK, TPTHLVGNYGHLVFRPNYYGPAGSQR, DLTNLWNQMTMDGKDNDWVEIWNSHGK, IPDAHFLSEAQLNGAK, TRGAHLINCTGACPHFVYTK, DSVLMLR, ITGVPAK, VDVK, DVRVDVK, ITPTHLVGNYGHLVFRPNYYGPAGSQR, VEIWNSHGK, DWVEIWNSHGK, KPWPTLTGR, VFIVYR, EADNNIAK, LAEDEAAAQYILDTAPHSK, VICR, EADVVAGGSK, LDLNVTAEHTYINMTEPAIKPMWESK, VIREEQSK, EADVVAGGSKDK, LKYPLIR, VSMWHTPELYMDLLEGSTQSVCPVR, EDEAAAQYILDTAPHSK EEQSKDIPPMPNIPELNPR, LQDGNTVEVRPVFEILK, VVSISPDFNSSTIK, EGVPYTPFQNYIVDK, LQRGGPCVEISPIDATAIGVK, VYSPLPGTAPVSFSAGHR, EIWNSHGK, LVGSEGK, WARDLTNLWNQMTMDGK, EQTDLPYLIR, LYDAHNLK, WIHPLPGTDGALALAMAHVIIK, EYATTQPAMIICGGGTMHWYYSDVLLR, LYDAHNLKEQTDLPYLIR, WQIFLALAK, FLREADVVAGGSK, MDSTALYSDVVLPSAHWYEK, WVEIWNSHGK, FLREADVVAGGSKDK, NHSPDCISVYSPLPGTAPVSFSAGHR, YDAHNLK, FNSPHSR, NLWPK, YIGATPISL, FNSPHSRHSIHSTFK, NQWSWDK, YIILWGANPTQTR, FKANWTSPMK, NQWSWDKK, YLMHAIDTR, FPFRFNSPHSR, NWLNQAK, YMDLLEGSTQSVCPVR, FQVK, NWTSPMK, YPLIR, FYDEQFK, PDAHFLSEAQLNGAK, YSPLPGTAPVSFSAGHR, FYIWDSK, PGTAPVSFSAGHR, YVLR, PGTDGALALAMAHVIIK |
| Perchlorate reductase beta subunit (58.5, 26-29, 564)d | AMHVGFIDDQESSVFK, NQPSELMDILIGR, REQDGLVVIHQEK, CIGCFPR, NVETAPGLGYPR, RFGVALPLHPEYGTEPNVFYVPPVLGPR, CIGCQTCTVACK, NVETAPGLGYPRNWQSK, RSADMMISPMT, DDQESSVFK, PFEFDYAGR, RQLAYVADLNK, DQESSVFK, PPVLGPR, SADMMISPMT, EQDGIVVIHQDK, PSELMDILIGR, TAPGLGYPR, ETAPGLGYPR, QLAYVADLNK, TLQAER, EVLK, IEKGVAPACVAECAGR, TLQAEREK, FGVALPLHPEYGTEPNVFYVPPVLGPR, IKNQPSELMDILIGR, TLWTSGPGQDYMYWR, GGGYKDGVLQK, IPPMIDYGVPFEFDYAGR, VEMPNGEHTADPK, GKIPPMIDYGVPFEFDYAGR, ISMTQLEQLFGK, YVADLNK, GVAPACVAECAGR, LFEGKPGR, GYKDGVLQK, MCNHCAKPACLEACPNEAIYK |
| Perchlorate reductase gamma subunit (26.7, 6, 6) | APFTPFSPAVDSK, KPLVDAGQNFDM, LDPK, ASAHAKAFDSLKPNAK, KPLVDAGQNFDMEK, SVTATGKSNPVHTHFK |
| Perchlorate reductase delta subunit (61.3, 9-14, 75) | DFLER, HLCAWLPLVR, QELGVCWGELLEHFNEVLAPGLKLACSR, DFLERHLCAWLPLVR, LAQKDFLER, RPNLLLELK, ELKEELGE, LVLAADK, SDRPNLLLELK, EVFESQFLSAFETNMPSPSASLNEGVHIFK, LVLAADKALCR, SHIYSLLASGFGYPDESGYQGFSDGK, FIDEIR, MNSITDDRLVLAADK, VTTPFFVTLAEFAESFALANLR, GFYSNFGLQVDSK, QAQAHLEGMSADAYK |
a
Results are the average of three mass spectrometric runs per sample. All the readings have a standard error of less than 12% of the mean.
b
Peptides also match with (UniProtKB protein database accession numbers in parentheses) Pseudomonas sp. PK (Q673K7), sequence coverage 24%; and Ideonella dechloratans (Q9F437), sequence coverage 2%.
c
Peptides also match with (UniProtKB protein database accession no. in parentheses) Dechlorosoma sp. KJ (B2D1S4), sequence coverage 19.8%; Dechlorosoma sp. PCC (A7LI70), sequence coverage 18.4%; and Dechloromonas sp. PC1 (A7LI69), sequence coverage 13.4%.
d
Peptides also match with Dechlorosoma sp. KJ (UniProtKB protein database accession no. B2D1S5), sequence coverage 16.8%.