FIG. 3.

Gene sequence for the xylC^m insert in plasmid pHsh-xylC^m (a) and SDS-PAGE analysis of total soluble proteins in recombinant E. coli JM109 cells harboring pHsh-xylC or pHsh-xylC^m (b). (a) Restriction sites, including the 3′ HindIII cloning sites, Hsh promoter sites (−10 and −35 regions), translation initiation region (70 nt), and transcription terminator sites, are illustrated. Oversized italic bases represent mutant nucleotides. rbs, ribosome binding site. (b) Lane M, low-molecular-weight marker; lane 1, E. coli JM109 containing the plasmid pHsh; lane 2, E. coli JM109 containing the plasmid pHsh-xylC; lane 3, E. coli JM109 containing the plasmid pHsh-xylC^m; lane 4, purified recombinant XylC^rec.