FIG. 6.

(a) Stereochemical analysis of reaction mixtures containing pNP-xyloside and XylC from T. saccharolyticum JW/SL-YS485. The enzymatic reaction was carried out at 40°C for 1, 2, 5, and 10 min. The migration times of β-xylose and β-xylopyranoside were 6.58 and 6.88 min, respectively. (b) Thin-layer chromatography of the transglycosylation products from pNP-xyloside (5 mM) and various alcohols in 50 mM morpholineethanesulfonic acid (MES) buffer (pH^65°C 6.0). Lane M, pNP-xyloside; lane 0, control (reaction mixture without alcohol); lane 1, plus methanol; lane 2, plus ethanol; lane 3, plus 1-propanol; lane X, standard xylose.