TABLE 2.
Purification of XylC from T. saccharolyticum strain JW/SL-YS485
| Purification step | Enzyme | Total protein (mg) | Total activity (U) | Sp act (U/mg)a | Purification (fold) | Recovery (%) |
|---|---|---|---|---|---|---|
| Crude extract | Mixture | 1,069 | 1,847 | 1.73 | 1.00 | 100 |
| DEAE-Sepharose | Mixture | 121 | 1,749 | 14.5 | 8.35 | 95 |
| Phenyl- | XylA | 51.0 | 894 | 17.5 | 12.2 | 56 |
| Sepharose | XylC | 39.0 | 140 | 3.59 | ||
| DEAE- | XylA | 11.6 | 334 | 28.9 | 22.5 | 21 |
| Sepharose | XylC | 5.88 | 59.6 | 10.1 | ||
| Gel filtration | XylA | 5.06 | 172 | 34.1 | 46.0 | 12 |
| XylC | 1.17 | 53.8 | 45.8 |
a
Activity was determined with p-nitrophenyl-β-d-xylopyranoside as substrate (1 mM) in 50 mM potassium hydrogen phthalate-imidazole buffer, pH 6.0, at 65°C.