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. 2010 Nov 15;55(2):688–695. doi: 10.1128/AAC.00666-10

FIG. 5.

FIG. 5.

Binding of C16(ω7)K-β12 to DNA and the resulting synthesis inhibition. (A) Agarose gel runs of bacterial plasmid pUC19 (200 ng) after incubation with various OAK concentrations (30 min, room temperature). (B) DNA restriction inhibition assay showing (from left to right) agarose gel runs (in duplicates) of pUC19 in its native state and after treatment with the DNase XbaI or when preincubated (30 min) with OAK (4× the MIC) and then treated with XbaI. The rightmost lanes show the gel runs of pUC19 after extraction from OAK-free and OAK-pretreated bacteria (4× the MIC). (C) Bacterial viability (CFU count) and macromolecule synthesis ([3H]thymidine incorporation assay) using E. coli CGSC 5895. Symbols: circles, untreated control; triangles, OAK-treated bacteria (4× the MIC). Open symbols, radioactivity signals; closed symbols, bacterial viability.