TABLE 1.
Strain or plasmid | Description | Reference or source |
---|---|---|
Strains | ||
E. coli | ||
DH5α | Cloning host | |
STBL2 | Cloning host | |
C600 ΔfolP::Kmr | folP mutant | 7 |
M. smegmatis mc2155 | ||
Plasmids | ||
pYUB854 | Cosmid vector | 3 |
phAE87 | Phasmid vector carrying full length DNA of mycobacteriophage PH101 | 3 |
pMV261 | E. coli-mycobacteria shuttle plasmid vector (multicopy in mycobacteria) | 13 |
pNN301a | pMV361-type integrative vector (single copy in mycobacteria) | 13; this study |
pNN301 has an int-attP fragment of mycobacteriophage L5 instead of oriM.