TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | Cloning host | |
| STBL2 | Cloning host | |
| C600 ΔfolP::Kmr | folP mutant | 7 |
| M. smegmatis mc2155 | ||
| Plasmids | ||
| pYUB854 | Cosmid vector | 3 |
| phAE87 | Phasmid vector carrying full length DNA of mycobacteriophage PH101 | 3 |
| pMV261 | E. coli-mycobacteria shuttle plasmid vector (multicopy in mycobacteria) | 13 |
| pNN301a | pMV361-type integrative vector (single copy in mycobacteria) | 13; this study |
a
pNN301 has an int-attP fragment of mycobacteriophage L5 instead of oriM.