TABLE 3.

Dapsone susceptibility of the recombinant M. smegmatis strains

Strain or mutation	Dapsone MIC (μg/ml)	Reference of footpad test
Wild type	0.5
48-1 (Val → Ile)	0.5
48-2 (Val → Leu)	0.5
48-3 (Val → Phe)	1.0
48-4 (Val → Asp)	—a
48-5 (Val → Ala)	1.0
48-6 (Va → Gly)	1.0
53-1 (Thr → Ala)	4.0	6
53-2 (Thr → Ile)	8.0	6, 10, 16
53-3 (Thr → Ser)	0.25
53-4 (Thr → Pro)	2.0
53-5 (Thr → Asn)	2.0
53-6 (Thr → Ser)	0.25
54-1 (Arg → Arg)	0.5
54-2 (Arg → Gly)	1.0
54-3 (Arg → Trp)	0.5
55-1 (Pro → Thr)	1.0
55-2 (Pro → Ala)	2.0
55-3 (Pro → Ser)	2.0
55-4 (Pro → His)	2.0
55-5 (Pro → Arg)	8.0	6, 16
55-6 (Pro → Leu)	4.0	6, 10

^aIsolation of an folP-disrupted M. smegmatis strain carrying the M. leprae folP1 with mutation 48-4 was unsuccessful.