FIG. 3.
Blastocystis subtype 7 exhibits a time-dependent increase in redox activity when cultured in a 96-well plate under the resazurin assay conditions described in this study. The starting parasite density was 0.5 × 106 cells in 200 μl of IMDM supplemented with 10% horse serum and 0.5% DMSO. The redox activity of the culture peaked at 24 h, followed by a steady decline. A drug contact duration of 24 h was chosen based on these results. Each point represents a mean of 6 values derived from two independent experiments, with each experiment conducted in triplicate. The error bars represent standard errors.