Skip to main content
. 2010 Nov 22;55(2):637–648. doi: 10.1128/AAC.00900-10

TABLE 3.

Statistical evaluation of the quality of resazurin and XTT assaysa

Assay Z′ factor Cv (%)b Linearity (R2)c
Resazurin 0.9 ± 0.02 7.6 ± 0.35 0.995
XTT 0.89 ± 0.1 2.4 ± 0.54 0.983
a

Ideal HTS parameters are a Z′ factor of >0.5 (74) and a Cv of <10% (30).

b

Cv %, coefficient of variance of cell controls.

c

Linearity of the dye reduction-versus-parasites/well curve.