TABLE 1.
Sequences of primers used for specific gene detection
| Targeta | Primer nameb | Primer sequence (5′ to 3′) | Size (bp) | Ta (°C)c | Source or reference |
|---|---|---|---|---|---|
| armA | armA-F | AGGTTGTTTCCATTTCTGAG | 405 | 51 | This work |
| armA-R | ACCTATACTTTATCGTCGTC | ||||
| rmtA | rmtA-F | CGTGACATAACATCTGTATG | 333 | 51 | This work |
| rmtA-R | TTCAAATTCATCAGGCAGTG | ||||
| rmtB | rmtB-F | ATTGGGATTTTACCTTTGCC | 290 | 51 | This work |
| rmtB-R | TATAAGTTCTGTTCCGATGG | ||||
| rmtC | rmtC-F | AGATACCAAATCCAACTACG | 369 | 51 | This work |
| rmtC-R | TAAGTAGAAGATCACTCTCG | ||||
| rmtD | rmtD-F | TCAAAAAGGAAAAGGACGTG | 500 | 51 | This work |
| rmtD-R | CGATGCGACGATCCATTC | ||||
| rmtD-F2d | ATGAGCGAACTGAAGGAAAAAC | 744 | 57 | This work | |
| rmtD-R2d | TCATTTTCGTTTCAGCACGTAAA | ||||
| npmA | npmA-F | CTCAAAGGAACAAAGACGG | 641 | 51 | 7 |
| npmA-R | GAAACATGGCCAGAAACTC | ||||
| blaTEM | tem1-F | ATGAGTATTCAACATTTTCGTG | 861 | 55 | This work |
| tem1-R | TTACCAATGCTTAATCAGTGAG | ||||
| blaSHV | shv1-F | ATGCGTTATATTCGCCTGTG | 861 | 55 | This work |
| shv1-R | TTAGCGTTGCCAGTGCTCG | ||||
| blaCTX-M | ctx-MU1 | ATGTGCAGYACCAGTAARGT | 803 | 55 | 13 |
| ctx-MU2 | TGGGTRAARTARGTSACCAG | ||||
| blaKPC | Uni-KPC-F | ATGTCACTGTATCGCCGTCT | 882 | 55 | 17 |
| Uni-KPC-R | TTACTGCCCGTTGACGCCC | ||||
| qnrB | qnrB-F | CCGACCTGAGCGGCACTGA | 523 | 55 | 18 |
| qnrB-R | CGCTCCATGAGCAACGATGCCT |
a
rmtE was reported after the conclusion of this work, and its analysis was not included.
b
F, forward; R, reverse.
c
Ta, PCR annealing temperature.
d
Primers to amplify the complete rmtD gene.