TABLE 2.

Ligase detection reaction with tagged allele-specific upstream primers ligated to conserved sequence downstream primers

Gene	LDR primerc	Sequencea	FlexMAP microsphere set IDb
pfmdr1 (region 1)	86N	5′-tacactttctttctttctttctttTTGGTGTAATATTAAAGAACATGA-3′	10
	86Y	5′-atcatacatacatacaaatctacaTTGGTGTAATATTAAAGAACATGT-3′	12
	Com 86	5′-phosphate-ATTTAGGTGATGATATTAATCCTA-biotin-3′
	184Y	5′-tcaaaatctcaaatactcaaatcaGCCAGTTCCTTTTTAGGTTTATA-3′	18
	184F	5′-ctacaaacaaacaaacattatcaaGCCAGTTCCTTTTTAGGTTTATT-3′	28
	Com 184	5′-phosphate-TATTTGGTCATTAATAAAAAATGCA-biotin-3′
pfmdr1 (region 2)	1034S	5′-ttacctttatacctttctttttacATGCAGCTTTATGGGGATTCA-3′	30
	1034C	5′-caatttcatcattcattcatttcaATGCAGCTTTATGGGGATTCT-3′	35
	Com 1034	5′-phosphate-GTCAAAGCGCTCAATTATTTATT-biotin-3′
	1042N	5′-cttttcatcttttcatctttcaatCCAAACCAATAGGCAAAACTATT-3′	37
	1042D	5′-ctttttcaatcactttcaattcatCAAACCAATAGGCAAAACTATC-3′	54
	Com 1042	5′-phosphate-AATAAATAATTGAGCGCTTTGAC-biotin-3′
	1246D	5′-tcatcaatcaatctttttcactttAATATATGTGATTATAACTTAAGAG-3′	59
	1246Y	5′-tcataatctcaacaatctttctttTAATATATGTGATTATAACTTAAGAT-3′	68
	Com 1246	5′-phosphate-ATCTTAGAAACTTATTTTCAATAG-biotin-3′

^aNucleotides in lowercase type represent TAG sequences added to the 5′ ends of each allele-specific LDR primer.

^bID, identification. Luminex microsphere sets are synthesized to exhibit unique fluorescence. Each microsphere set is coupled to different anti-TAG sequences that are complementary to allele-specific TAG sequences.

^cCom, common (conserved) sequence primer positioned immediately downstream from the allele-specific primer.