TABLE 1.
Primers used in this study
| Primer | Sequence (5′-3′) | Amplicon length (bp)a | % primer efficiency (E)a,b |
|---|---|---|---|
| acrA-F | CTCTCAGGCAGCTTAGCCCTAA | 107 | 95 |
| acrA-R | TGCAGAGGTTCAGTTTTGACTGTT | ||
| acrB-F | GGTCGATTCCGTTCTCCGTTA | 107 | 95 |
| acrB-R | CTACCTGGAAGTAAACGTCATTGGT | ||
| rpsL-F | GCAAAAACGTGGCGTATGTACTC | 104 | 97 |
| rpsL-R | TTCGAAACCGTTAGTCAGACGAA | ||
| mdfA-F | CATTGGCAGCGATCTCCTTT | 103 | 97 |
| mdfA-R | TTATAGTCACGACCGACTTCTTTCA | ||
| norE-F | CTGGCGGCAGCGGTAA | 108 | 94 |
| norE-R | TGCCATACAGACACCCACCATA | ||
| tolC-F | AAGCCGAAAAACGCAACCT | 100 | 95 |
| tolC-R | CAGAGTCGGTAAGTGACCATC |
a
Amplicon lengths and primer efficiencies in the rows for forward (F) primers correspond to the respective primer pairs.
b
As measured by the efficiency equation, E = 10(−1/m) − 1, where m is ΔCT/Δ[cDNA].