HPLC analysis of the reaction product without enzymes (A, C, E, and G) and with recombinant Acel_0264 (B and D) and Acel_0106 (F and H). Adenosine (A and B) (positive control), AFL (C, D, E, and F), and FL (G and H) were used as substrates. Time (in minutes) is shown on the x axes, and optical density (in milliabsorbance units [mAU]) is shown on the y axes.