TABLE 3.
Efficiency of PB1 peptides carrying affinity-enhancing amino acid substitutions to compete with PA bindinga
| Peptide | Amino acid sequence | Mean IC50 (nM) ± SD |
|---|---|---|
| PB11-15M1Y | YDVNPTLLFLKVPAQ | 12.7 ± 3.7 |
| PB11-15D2N | MNVNPTLLFLKVPAQ | 45.2 ± 8.3 |
| PB11-15V3Y | MDYNPTLLFLKVPAQ | 6.3 ± 2.1 |
| PB11-15T6Y | MDVNPYLLFLKVPAQ | 21.64 ± 1.5 |
| PB11-15P13R | MDVNPTLLFLKVRAQ | 11.4 ± 1.7 |
| PB11-15P13K | MDVNPTLLFLKVKAQ | 16.6 ± 5.0 |
| PB11-15A14I | MDVNPTLLFLKVPIQ | 21.7 ± 9.3 |
| PB11-15A14L | MDVNPTLLFLKVPLQ | 36.3 ± 14.8 |
| PB11-15A14V | MDVNPTLLFLKVPVQ | 22.2 ± 5.4 |
| PB11-15Q15V | MDVNPTLLFLKVPAV | 52.2 ± 16.2 |
| PB11-15Q15F | MDVNPTLLFLKVPAF | 13.9 ± 2.8 |
a
Determination of the IC50s of PB11-15 peptides harboring the affinity-enhancing amino acid substitutions by competitive ELISA using cell extract containing HA-tagged PA of A/WSN/33 and increasing concentrations of competitive peptides. Amino acid replacements compared to the wt are indicated in boldface.