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Real-time quantitative PCR (RT-qPCR) of histones in treated and untreated parasites. Relative transcript abundance (in comparison to that of RH under control conditions) for all known T. gondii histone subunits was determined by RT-qPCR for both parental (RH) and TgMSH1-deficient (Tgmsh-1mut) strains after exposure to monensin (0.75 ng/ml, 24 h). Each bar represents the mean value for three independent replicates. Error bar = 1 standard deviation.
