FIG. 3.
Flow cytometry analysis of parental (RH) and TgMSH1-deficient (Tgmsh-1mut) T. gondii strains in response to monensin. Intracellular parasites were exposed to either normal culture medium or culture medium plus 0.75 ng monensin/ml for 24 h. DNA content was measured using Sytox green. (A) Representative histograms are shown. Each histogram represents 10,000 total events. (B) Percentage ± standard deviation of parasites in G1 or S/M phases determined by gating for three separate experiments is indicated in the bar graphs.