TABLE 1.

Microarray analysis shows that exposure to monensin causes significant downregulation in transcription of Toxoplasma genes^a

GeneID	Function/homology	Fold downregulation
TGME49 017740	Oxoacyl-ACP reductase	33.6
TGME49 090200	Opine dehydrogenase	24.4
TGME49 055450	Hypothetical protein	20.6
TGME49 055450	FUN14 family domain-containing protein	18.8
TGME49 094640	Ribonucleoside-diphosphate reductase, large subunit	18.8
TGME49 039530	Alanine-glyoxylate aminotransferase	18.1
TGME49 097120	Hypothetical protein	16.1
TGME49 120110	Proliferating cell nuclear antigen	16.1
TGME49 045670	Pyruvate dehydrogenase	16.0
TGME49 043200	Hypothetical protein	15.6
TGME49 067140	SRS38B	15.1
TGME49 018540	Hypothetical protein	14.1
TGME49 063500	Vacuolar protein sorting 26	14.0
TGME49 069870	Hypothetical protein	13.7
TGME49 062730	Rhoptry kinase family protein ROP16	12.8
TGME49 116280	Hypothetical protein	12.2
TGME49 097860	Hypothetical protein	12.1
TGME49 064080	Acyl carrier protein	11.9
TGME49 025050	Adenosylhomocysteinase	11.9
TGME49 007060	Ribonucleotide-diphosphate reductase, small subunit	11.8
TGME49 105510	Hypothetical protein	11.6
TGME49 018270	Hypothetical protein	11.4
TGME49 040630	Protein kinase	11.4
TGME49 116260	Hypothetical protein	11.3
TGME49 085860	SRS20C	11.3
TGME49 007140	SRS49B	11.2
TGME49 039750	Hypothetical protein	11.0
TGME49 009160	Glycylpeptide N-tetradecanoyltransferase	11.0
TGME49 118430	Malate dehydrogenase	11.0
TGME49 024570	Hypothetical protein	10.9

^aShown are the 30 genes with the largest transcriptional downregulation in response to monensin exposure (2.5 ng/ml for 24 h). ACP, acyl carrier protein.