FIG. 3.
VAN-allograft resists colonization by S. aureus. (a) Allografts were challenged with 10 to 105 CFU for 6 h and adherent S. aureus collected. Colonization of control bone and VAN-allograft increased as a function of inoculum, with VAN-allograft values consistently lower than those measured for control allograft. *, P < 0.05. (b) Control allograft or VAN-allograft was challenged with S. aureus (Ci = 104 CFU/ml) for 2, 5, 8, or 12 h, and numbers of bacteria adherent to the surface of the bone were assessed by direct colony counts, shown as a percentage of the control value for each time period. At all times, numbers of adherent bacteria recovered from the VAN-allograft were smaller than those from the control. Values shown are mean ± SEM for three separate determinations in one representative experiment. *, P < 0.05.