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Effect of time (up to 180 min), temperature (37°C versus 4°C), and presence of unlabeled drug (500 μM) on the uptake of radiolabeled artemisone in uninfected erythrocytes. Cell samples were divided into supernatant (open bars) and pellet (closed bars) fractions after lysis in Triton and deproteinization with TCA. The initial concentration of artemisone was 20 μM. Data are derived from 3 independent experiments and are shown as means ± SEM.
