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. 2010 Dec 6;55(2):550–556. doi: 10.1128/AAC.01216-10

FIG. 3.

FIG. 3.

Effect of time (up to 90 min), temperature (37°C versus 4°C), and presence of unlabeled drug (100 μM) on the uptake of radiolabeled artemisone in mature infected erythrocytes. Cell samples were divided into supernatant and pellet fractions after lysis in Triton and deproteinization with TCA, with the data presented for the pellet fraction only. Data are corrected for 100% parasitemia. The initial concentration of artemisone was 0.4 μM. Circles, 37°C; triangles, 4°C; squares, 37°C and in the presence of unlabeled artemisone (100 μM); inverted triangles, 4°C and in the presence of unlabeled artemisone (100 μM). Data are derived from 3 independent experiments and are shown as means ± SEM.