FIG. 5.

Phagocytosis of H. capsulatum agglutinates by macrophages, normalized by phagocytosis levels obtained with yeast incubated with an isotype control. (A) MAb to Hsp60 increased phagocytosis of H. capsulatum yeast, with a greater efficacy for smaller aggregates than larger ones. (B) A blockade of macrophage CR3 receptors reduced the phagocytosis of larger agglutinates compared to that of smaller ones. (C) CD11b^−/− macrophages are less able to phagocytose larger agglutinates, and results were similar to those obtained from the CR3 receptor blockage with antibodies. (D) A blockade of Fc receptors reduced the phagocytosis of smaller aggregates compared to that of large ones. The results shown are averages from three independent experiments.