TABLE 1.

Primer sequences, product sizes, and melting temperatures used for q-RT-PCR of designated target genes

Genea	GenBank accession no.	Primer sequence (5′→3′)	Product size (bp)	Product Tmb
HPRT	NM_001003357	CGC TGA GGA TTT GGA AAA AG	150	82.5
		AAT CCA GCA GGT CAG CAA AG
TNF-α	NM_001003244	TGC CTG CTG CAC TTT GG	125	84.0
		GCT ACT GGC TTG TCA CTT GG
MMP-1	XM_546546	CGC GTA AAT CCC TTC TAT CC	157	80.5
		CAT CCT GAC CCT GAA CAA CC
MMP-2	XM_535300	TGG AGC AAG AAC AAG AAG ACC	182	82.0 + 85.5
		CCC TTG AAG AAG TAG CTA TGA CC
MMP-3	NM_001002967	ACA CCA GCT GCA TGT GAC C	123	82.0
		GAA CCC AGG TTC AAG TGT CC
HS3ST3A1	XM_546631	TCA TCG GCG TGA AGA AGG	200	91.0
		GCG GGT GAC AAA GTA ACT GG
IL-1β	NM_001037971	CAG GAC ATA AGC CAC AAA TAC C	109	80.0
		CAA AGC TCA TGT GGA ACA CC
HS2	XM_539153.2	TTC AGA CAC CAT GCT TGA CC	142	59.5
		TCT CAC ACT GCT GAG GAA GG

^aHPRT, hypoxanthine phophoribosyltransferase, TNF-α, tumor necrosis factor alpha; MMP-1, matrix metalloproteinase 1; MMP-2, matrix metallopeptidase 2; MMP-3, matrix metallopeptidase 3; HS3ST3A1, heparan sulfate d-glucosaminyl 3-O-sulfotransferase 3A1; IL-1β, interleukin-1β; HS2, hyaluronan synthase 2.

^bT_m, melting temperature.