TABLE 1.
Genes whose expression is altered by glucose or inactivation of CcpA or LacD.1
| Gene code | Functional categorya (no. of genes in categoryb) | No. (%) of genes with differential expressionc |
|||||
|---|---|---|---|---|---|---|---|
| Glucose |
CcpA |
LacD.1 |
|||||
| Increased | Decreased | Increased | Decreased | Increased | Decreased | ||
| J | Translation (131) | 15 (11.5) | 5 (3.8) | 4 (3.1) | 0 | 1 (0.8) | 1 (0.8) |
| K | Transcription (65) | 3 (4.6) | 9 (13.8) | 8 (12.3) | 1 (1.5) | 0 | 1 (1.5) |
| L | Replication, recombination, repair (93) | 3 (3.2) | 2 (2.2) | 5 (5.4) | 0 | 2 (2.2) | 0 |
| D | Cell cycle, cell division, chromosome partitioning (14) | 1 (7.1) | 0 | 0 | 0 | 0 | 0 |
| V | Defense mechanisms (15) | 1 (6.7) | 4 (26.7) | 2 (13.3) | 1 (6.7) | 0 | 0 |
| T | Signal transduction mechanisms (30) | 0 | 5 (16.7) | 6 (20.0) | 0 | 0 | 0 |
| M | Cell wall, membrane, envelope biogenesis (51) | 4 (7.8) | 2 (3.9) | 0 | 0 | 0 | 0 |
| N | Cell motility (6) | 1 (16.7) | 1 (16.7) | 0 | 0 | 1 (16.7) | 0 |
| O | Posttranslational modification, protein turnover, chaperones (42) | 2 (4.8) | 3 (7.1) | 4 (9.5) | 0 | 0 | 0 |
| C | Energy production and conversion (50) | 2 (4.0) | 18 (36.0) | 19 (38.0) | 1 (2.0) | 0 | 5 (10.0) |
| G | Carbohydrate transport and metabolism (78) | 7 (9.0) | 58 (74.4) | 49 (62.8) | 5 (6.4) | 1 (1.3) | 3 (3.8) |
| E | Amino acid transport and metabolism (76) | 5 (6.6) | 10 (13.2) | 14 (18.4) | 5 (6.6) | 5 (6.6) | 6 (7.9) |
| F | Nucleotide transport and metabolism (53) | 6 (11.3) | 7 (13.2) | 8 (15.1) | 2 (3.8) | 1 (1.9) | 2 (3.8) |
| H | Coenzyme transport and metabolism (36) | 0 | 4 (11.1) | 3 (8.3) | 0 | 1 (2.8) | 0 |
| I | Lipid transport and metabolism (34) | 0 | 2 (5.9) | 2 (5.9) | 0 | 0 | 6 (17.6) |
| P | Inorganic ion transport and metabolism (47) | 2 (4.3) | 4 (8.5) | 4 (8.5) | 1 (2.1) | 1 (2.1) | 0 |
| Q | Secondary-metabolite biosynthesis, transport, and catabolism (10) | 0 | 0 | 1 (10) | 0 | 0 | 0 |
| R | General function prediction only (127) | 6 (4.7) | 15 (11.8) | 12 (9.4) | 2 (1.6) | 0 | 1 (1.6) |
| S | Unknown function (122) | 4 (3.3) | 8 (6.6) | 7 (5.7) | 5 (4.1) | 2 (1.6) | 1 (0.8) |
| Uncategorized (hypothetical) | 8 | 25 | 26 | 2 | 22 | 1 | |
| Total | 70 | 182 | 174 | 25 | 37 | 27 | |
Genes were categorized according to a functional classification system, COGs (clusters of orthologous groups) (43, 44).
Number of genes in the assigned category in the S. pyogenes SF370 genome.
Genes displaying a change in expression of >2-fold that was statistically significant (P < 0.05) by ANOVA across all experiments. Gene expression was compared as follows: for glucose, wild-type strain (HSC5) in C medium supplemented with 1% glucose/wild-type strain in unsupplemented C medium in exponential growth; for CcpA, CcpA− strain (CKB206)/wild-type strain in C medium supplemented with glucose in exponential growth; for LacD.1, LacD.1− strain (JL151)/wild-type strain in C medium supplemented with glucose in stationary-phase growth.