TABLE 2.
Genea | Ratio of transcript abundanceb |
|||||
---|---|---|---|---|---|---|
Glucose |
CcpA |
LacD.1 |
||||
Array | RT-PCR | Array | RT-PCR | Array | RT-PCR | |
lctO | 0.047 | 0.010 | 43.5 | 388.1 | NSc | 1.31 |
malM | 0.33 | 0.28 | 1.3 | 1.6 | NS | |
ackA | 0.63 | 0.77 | 2.8 | 3.7 | NS | |
xpt | 2.5 | 10.7 | 0.361 | 0.31 | 1.8 | |
dexS | 0.082 | 0.22 | 0.17 | 0.20 | NS | |
SPy_1507 | NS | 1.4 | 1.76 | 3.7 | 4.6 | |
arcA | 0.044 | 19.7 | 35.6 | 0.055 | 0.111 |
The locus tags corresponding to the genes are as follows: for lctO, SPy_0414; for malM, SPy_1292; for ackA, SPy_0109; for xpt, SPy_1136; for dexS, SPy_2096; and for arcA, SPy_1547.
For glucose, the ratio of transcript abundance in the wild-type strain (HSC5) in C medium plus glucose to that in the wild-type strain in C medium alone; for CcpA, the ratio of transcript abundance in the CcpA− strain (CKB206) in C medium plus glucose to that in the wild-type strain in C medium plus glucose; for LacD.1, the ratio of transcript abundance in the LacD.1− strain (JL151) in C medium plus glucose to that in the wild-type strain in C medium plus glucose.
NS, not significant by ANOVA.