FIG. 4.

Antibacterial activities of rmPGLYRP-1 against Gram-positive and Gram-negative bacteria. (A to C) An assay mixture for antibacterial activity contained 1 × 10⁶ CFU of S. aureus (A), S. epidermidis (B), and L. monocytogenes (C) rmPGLYRP-1 or bovine serum albumin (BSA) as a control in 1 ml of the assay buffer with or without anti-mPGLYRP-1 antibody (1 mg/ml). After 0.5, 1, 3, and 6 h of incubation, 100 μl of the mixture was taken and plated on tryptic soy agar plates after serial dilution. Each result was obtained from six independent assays. An asterisk indicates that a value is significantly different from the value obtained from BSA controls at P < 0.01. (D, E) An assay mixture for antibacterial activity contained 1 × 10⁶ CFU of S. aureus (D) and L. monocytogenes (E) rmPGLYRP-1 or BSA as a control in 1 ml of the assay buffer with or without 1 mM zinc chloride or 1 mM EDTA. Each result was obtained from six independent assays. An asterisk indicates that a value is significantly different from the value obtained from BSA controls at P < 0.01.