FIG. 4.
Immune responses elicited by mucosal immunization with rZmpB. The mice were intranasally immunized with rZmpB, with CT as the mucosal adjuvant. (A) The levels of specific total IgG, IgG1, IgG2a, IgG2b, IgG3, or IgA against rZmpB in sera or saliva samples were tested by ELISA. The antibody titers were defined as the reciprocal of the dilution of sera giving a 2.1-fold value of the highest absorbance value versus the background level at 450 nm. The antibody titers were measured 7 days after the third immunization. (B) Effect of mucosal immunization with rZmpB on cytokine production in splenocytes. Seven days after the last immunization, the splenocytes (1 × 105 cells/well) were cultured in the absence (uninduced) or in the presence (induced) of 5 μg of rZmpB for 72 h at 37°C. After 72 h, the culture supernatants were assayed for the levels of IFN-γ, IL-10, and IL-17A by ELISA. *, P < 0.05 compared between groups denoted by horizontal lines.