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Protection against pneumococcal pneumonia by mucosal immunization with rZmpB. Mice immunized with rZmpB by the nasal route were intranasally challenged with pneumococcal strain CMCC 31693 (1.0 × 107 CFU) (A) or CMCC 31614 (1.5 × 106 CFU) (B). The lung colonization of individual mice was determined at day 3 after challenge. Each dot represents one mouse. The horizontal lines indicate the median CFU per lung. ***, P < 0.001 compared between groups denoted by horizontal lines.
