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P. aeruginosa traversal of multilayered human corneal epithelial cells in vitro. PAO1 (106 CFU) was added to the apical compartment, and viable counts were taken from the apical (white bar) and basal (black bar) compartments at 2, 4, 6, and 8 h postinoculation. P. aeruginosa traversal was detected at each time point. Data from one of three independent experiments are shown.
