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. 2010 Dec 6;79(2):774–785. doi: 10.1128/IAI.00482-10

FIG. 1.

FIG. 1.

Interaction of two B. burgdorferi sensu stricto strains, N40 and B31, and of Osp antigens with human primary keratinocytes (KCs). The secretion of two proteins, the chemokine IL-8, and the AMP hBD-2, was measured by ELISA. (A and B) Comparison of the inflammatory responses of KCs induced by B. burgdorferi strains N40 and B31 for 24 h. Results from titration of Borrelia are shown as follows: 100 Borrelia cells/cell (100B), MOI of 100:1; 10B, MOI of 10:1; and 1B, MOI of 1:1. (C and D) Inflammatory response of KCs stimulated with lipidated (L-) OspC or OspA or unlipidated (U-) OspC or OspA for 6 and 24 h at a concentration of 100 ng/ml and Pam3Cys at a concentration of 10 μg/ml. (E and F) Inhibition by I. ricinus crude salivary gland extract (SG; 20 μg/ml) of the inflammation induced by the lipidated OspC or OspA, measured at 24 h. #, complete inhibition; φ, unstimulated cells; ***, P < 0.001; **, P < 0.01; and *, P < 0.05.