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MMP-2 activity in 24-h supernatants of PDL cell cultures (PDL) following a 2-h challenge with T. denticola. Gelatin zymogram (top) and Western immunoblot (bottom) probed with an anti-MMP-2 catalytic domain antibody. Lanes: C, PDL no-challenge control; Td, PDL challenged with T. denticola 35405; TdP, PDL challenged with T. denticola P0760; TdM, PDL challenged with T. denticola MHE. The locations of T. denticola protease (Td pr) and MMP-2 are indicated.
