FIG. 4.

MMP-2-specific inhibitor blocks MMP-2 activation and inhibits FN fragmentation. Lanes contain PDL culture supernatant alone (PDL) or after 2 h of treatment with T. denticola 35405, washing, and addition of fresh medium (PDL + Td). ARP101 was added to the fresh medium at the indicated concentrations. (A) Gelatin zymogram with positions of T. denticola protease complex (Td pr) and MMP-2 bands indicated. (B) Western immunoblot probed with anti-MMP-2 catalytic domain antibody. (C) Western immunoblot probed with an anti-FN antibody. Molecular weight standards (in thousands) are indicated.