FIG. 6.

Preferential recruitment of iNOS-producing CD11c^Lo-Int CD11b⁺ iDCs to spleens infected with ΔyopM Y. pestis KIM5. C57BL/6 mice were infected as described in the legend for Fig. 1 and analyzed for inflammatory leukocyte populations in livers and spleens. Shown are time courses of changes in populations of iDCs (distinguished as illustrated in Fig. 5) in spleens and livers during infection with parent (filled circles) and ΔyopM (open circles) Y. pestis. PMNs were gated out by removing CD11b⁺ Ly6G⁺ cells (upper right quadrants of scatter plots for Ly6G versus CD11b in Fig. 1B). The remaining live cells were evaluated for the presence of CD11b (myeloid cells) and CD11c (DCs). iDCs, inflammatory DCs (CD11b⁺ CD11c^Lo-Int). The point at time zero indicates the value for uninfected mice. Each datum point represents the average of values from 20 mice. The error bars indicate the standard deviations. Significant differences by unpaired Student's t test comparing data from mice infected with the parent and mutant strains are indicated (**, P < 0.01).