FIG. 8.

Inhibition of iMO/iDC recruitment into spleen and into circulation by parent Y. pestis KIM5. C57BL/6 mice were infected with parent and ΔyopM Y. pestis and analyzed by flow cytometry for populations of live leukocytes in spleen and peripheral blood on days 2 and 3 p.i. (indicated, respectively, as D2 and D3). P, mice infected with the parent strain; M, mice infected with the ΔyopM mutant. (A) Ly6G⁻ CD11b⁺ cells, distinguished as illustrated in the lower right quadrants of Fig. 1B. (B) Ly6G⁺ CD11b⁺ cells (PMNs), distinguished as illustrated in the upper right quadrants of Fig. 1B. Each datum point represents the average of values from 9 mice. The error bars indicate the standard deviations. Significant differences by unpaired Student's t test comparing data from mice infected with the parent and mutant strains are indicated (**, P < 0.01).