Y. pestis strains |
|
|
KIM5 |
Pgm− Lcr+ pCD1 pMT1 pPCP1; conditionally virulent Δpgm 2.MED strain; also called KIM D27 |
R. R. Brubaker |
KIM5-3003 |
ΔlacZ derivative of KIM5; 1.2 kb of lacZ coding sequence deleted by allelic exchange with pLacZ3 |
This study |
CO92.S6 |
CO92 Apr Pgm+ Lcr+ pFra pPCP1; reconstituted virulent strain made by introduction of pCD2Ap into CO92.S1 |
12 |
CO92.S19 |
Reconstituted Lcr+ ΔyopM2 strain made by introducing pCD2Ap ΔyopM2 from CO99-3015.S11 into CO92.S1 |
66 |
E. coli strain |
|
|
DH5αλpir
|
recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1deoR Δ(lacZYA-argF)U169 λpir+; host strain for maintaining pLacZ3 |
Lab stock |
Plasmid |
|
|
pLacZ3 |
Suicide allelic exchange plasmid for transferring internal 1.2-kb deletion into Y. pestis lacZ; 12.8 kb, R6K origin, SucB+ Smr ΔlacZ
|
4 |