TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant properties | Source or reference |
|---|---|---|
| Y. pestis strains | ||
| KIM5 | Pgm− Lcr+ pCD1 pMT1 pPCP1; conditionally virulent Δpgm 2.MED strain; also called KIM D27 | R. R. Brubaker |
| KIM5-3003 | ΔlacZ derivative of KIM5; 1.2 kb of lacZ coding sequence deleted by allelic exchange with pLacZ3 | This study |
| CO92.S6 | CO92 Apr Pgm+ Lcr+ pFra pPCP1; reconstituted virulent strain made by introduction of pCD2Ap into CO92.S1 | 12 |
| CO92.S19 | Reconstituted Lcr+ ΔyopM2 strain made by introducing pCD2Ap ΔyopM2 from CO99-3015.S11 into CO92.S1 | 66 |
| E. coli strain | ||
| DH5αλpir | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1deoR Δ(lacZYA-argF)U169 λpir+; host strain for maintaining pLacZ3 | Lab stock |
| Plasmid | ||
| pLacZ3 | Suicide allelic exchange plasmid for transferring internal 1.2-kb deletion into Y. pestis lacZ; 12.8 kb, R6K origin, SucB+ Smr ΔlacZ | 4 |