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Analysis of HPV-16 integration patterns. (A) Schematic of the distinctive structure of viral transcripts derived from intact HPV-16 plasmid genomes. (B) Structures of viral transcripts (type A or B) derived from either type I or type II integrated HPV DNA. (C) Example of 3′RACE (APOT) analysis of resolved amplicons derived from total RNA extracted from SiHa, HPV-16 W12E, HPV-16/HFK, primary (HPV-negative) HFK cells, and HNC tumor biopsy specimens T1 and T2.
