TABLE 1.
HPV integration status in HNC tumor tissues
| Tumor | HPV DNAa | mRNA splice structureb | GenBank accession no.b | Chromosome no. | Gene locusb |
|---|---|---|---|---|---|
| T1 | I | 880^human chromosome 13 | AL354696.11 | 13 | MTRF-1 |
| T2 | I | 880^human chromosome 6 | AL353138.10 | 6 | HNRPA3 |
| T3 | I | 880^human chromosome 13 | L11910.1 | 13 | Rb gene |
| T4 | I | 880^human chromosome 11 | AC009635.5 | 11 | Unknown |
| T5 | I | 880^human chromosome 7 | AC002467 | 7 | Unknown |
| T6 | I | 880^human chromosome 6 | AL135839 | 6 | SGK1 |
| T7 | E | 880^3358 HPV | |||
| T8 | E | 880^3358 HPV | |||
| T9 | E | 880^3358 HPV |
a
Physical status (I, integrated; E, extrachromosomal) of HPV DNA, determined by Southern blotting as illustrated in Fig. 1C.
b
Chimeric mRNA-derived amplification products were sequenced to determine mRNA structure (type A) and compared to the NIH GenBank database by BLASTN search to identify chromosomal integration loci: HNRPA3, heterogenous ribonucleoprotein A3; SGK1, serum glucocorticoid regulated kinase 1; MTRF1, mitochondrial translation release factor.