FIG. 2.

Expression analysis of pUL69 and its betaherpesviral homologs. (A and B) HEK293T cells were transfected with plasmids encoding N-terminally FLAG-tagged (A) or Myc-tagged (B) versions of pUL69 (lane 2), pC69 (lane 3), pRh69 (lane 4), pM69 (lane 5), and pU42 of HHV6 (lane 6) and ElHV1 (lane 7), as indicated. Transfection of an empty vector served as a specificity control (mock, lanes 1). Cells were lysed under denaturing conditions at about 36 h posttransfection. Lysates were separated by SDS-PAGE, and protein expression was analyzed by Western blotting using anti-FLAG- or anti-Myc-specific antibodies. Molecular size markers are indicated (kDa).